Histochemical Studies on Glycogen and Certain Enzymes in the Recovery of the Brain Injuries

要旨

In the recovery of brain injuries alteration of several enzymes of the cerebral cortices of rats was observed histochemically. Phosphatases were stained with GOMORI-TAKAMATSU's method as modified by SHIMIZU-ARIZONO, succinic dehydrogenase with the SELIGMAN-RUTENBURG's method as modified by ROSA-VELARDO, cytochrome oxidase with NADI reaction, 5-nucleotidase with the GOMORI technique and phosphorylase with TAKEUCHI-KURIAKI's method.Marked activity of alkaline phosphatse was shown from 2 days in the neuropil and capillary walls at a short distance from the wound and the intercellular ground substance in the adhered wound presented activity from 7 days.Evident activity of acid phosphatase was observed from 2 days in many glial cells surrounding the wound and from 7 days in the intercellular groung substance and glial cells in the adhered portions of the wound.The neuropil around the stab wound showed from 3 days a considerably incerased activity for 5-nucleotidase and the intercellular ground substance of the adhesion of the wound from 7 days.The above stated enzymes subsequently gave a gradual decerase of activity with the recovery of the injuries.At one days after operation, the activity of succinic dehydrogenase was slight or negative in the inner layer directly adjacent to the wound, the outer layer (surrounding the former) exhibiting a slightly, weaker activity than the unaffected nerve tissues.A slight or negative activity of cytochrome oxidase was demonstrated in the inner layer similarly to succinic dehydrogenase.A general increase in enzyme activity of succinic dehydrogenase and cytochrome oxidase was observed with the recovery of the injuries.The region containing glycogen similary exhibited a phosphorylase activity.

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