Serological analysis on the change of protein in scaly leaf of tulip bulb caused by low temperature treatment

要旨

It was examined by a serological method whether the change in the protein-pattern of scaly leaf of tulip bulb can be utilized as a signal in receiving the proper intensity of low temperature treatment in the bulb stage, or not. By using precipitation obtained from the antigen-antibody reaction effected by the double diffusion method according to Ouchterlony, six precipitin bands were detected in Mozart, and eight precipitin bands in Zwaneburg. It was found that the low temperature treatment reduces the protein level of bulb scales in both varieties. This reduction was due to the disapperance and decrease in the quantity of some special proteins.It was confirmed also in the result of analysis by immunoelectrophoresis that the disapperance of special proteins occurs with the low temperature treatment of bulbs.It was suggested from the above result that pursing the change in the protein of scaly leaf due to the low temperature treatment of bulbs by the serological method, it is possible to obtain signals for determining the proper intensity of low temperature treatment and further to establish the standard for selecting suitable varieties in the forcing culture.

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